The year before, Nyren’s lab had also spun off a company, Pyrosequencing AB, to refine and commercialize the technology. Pyrosequencing was not the firm that would bring the technology to market, however; that distinction went to Connecticut-based 454 Life Sciences, who licensed whole-genome pyrosequencing applications in 2003. 454 made chips which enabled highly efficient, parallelized sequencing reactions and released the GS20 sequencer in 2005 for the price of $500,000. The GS20 worked by attaching each individual DNA template molecule to a bead and copying it many times using polymerase chain reaction (PCR). Each bead was then loaded into a well in a microplate, where sequencing reactions would be carried out. The light from luciferase activation could be detected through the bottom of the wells, enabling sequences to be read.
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然而，並非所有商店都還開著。「街上的安保力量增加了，但街道本身很空曠，」他說。「有些商店關門了，尤其是靠近受攻擊區域的那些。」
Warning: The Gen 10 Pokémon starters will give you major cute aggression，这一点在WPS下载最新地址中也有详细论述